process¶
Calibrate and deskew an acquisition, optionally deconvolving it. Saves uint16 output beside the acquisition by default.
| Option | Description |
|---|---|
--deconvolve |
Apply RLGC deconvolution before deskewing. |
--flatfield-correction |
Correct uneven illumination. |
--save-float32 |
Preserve fractional intensities in float32 output. |
--skip-empty-below VALUE |
Zero channel tiles with insufficient signal above this intensity. |
--skip-empty-min-signal-fraction VALUE |
Required fraction above the cutoff; default 0.01. |
--resume |
Continue compatible completed tiles; otherwise overwrite output. |
--output PATH |
Set the output directory. |
--live PATH |
Process during acquisition using a supplied CYX illumination TIFF; pass the acquisition directory. |
--time-range START STOP |
Select timepoints. |
--pos-range START STOP |
Select positions. |
--z-downsample-level VALUE |
Deskewed Z reduction factor; default 2. |
--crop-after-deskew |
Crop Y to the region supported across all Z planes. |
--no-max-projection |
Disable per-tile and stage-placed maximum-Z images. |
--no-create-fused-max-projection |
Disable the stage-placed maximum-Z mosaic. |
--write-fused-max-projection-tiff |
Also save that mosaic as OME-TIFF. |
--decon-crop-scan VALUE |
Set scan planes per deconvolution chunk; default automatic. |
--decon-gpu-id VALUE |
Select the CUDA device; default 0. |
--decon-verbose VALUE |
Set solver verbosity; 0 suppresses iteration reports. |
--decon-fallback-step-scan VALUE |
Set chunk reduction after GPU allocation failure. |
--decon-psf-paths PATH |
Supply channel-ordered PSFs; repeat for each channel. |
--decon-scan-upsample VALUE |
Experimental 3D scan upsampling; requires deconvolution and full-volume GPU memory. |
--eager-mode |
Enable eager stopping for planar deconvolution. |
Append --help to list all options and defaults.