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process

Calibrate and deskew an acquisition, optionally deconvolving it. Saves uint16 output beside the acquisition by default.

Option Description
--deconvolve Apply RLGC deconvolution before deskewing.
--flatfield-correction Correct uneven illumination.
--save-float32 Preserve fractional intensities in float32 output.
--skip-empty-below VALUE Zero channel tiles with insufficient signal above this intensity.
--skip-empty-min-signal-fraction VALUE Required fraction above the cutoff; default 0.01.
--resume Continue compatible completed tiles; otherwise overwrite output.
--output PATH Set the output directory.
--live PATH Process during acquisition using a supplied CYX illumination TIFF; pass the acquisition directory.
--time-range START STOP Select timepoints.
--pos-range START STOP Select positions.
--z-downsample-level VALUE Deskewed Z reduction factor; default 2.
--crop-after-deskew Crop Y to the region supported across all Z planes.
--no-max-projection Disable per-tile and stage-placed maximum-Z images.
--no-create-fused-max-projection Disable the stage-placed maximum-Z mosaic.
--write-fused-max-projection-tiff Also save that mosaic as OME-TIFF.
--decon-crop-scan VALUE Set scan planes per deconvolution chunk; default automatic.
--decon-gpu-id VALUE Select the CUDA device; default 0.
--decon-verbose VALUE Set solver verbosity; 0 suppresses iteration reports.
--decon-fallback-step-scan VALUE Set chunk reduction after GPU allocation failure.
--decon-psf-paths PATH Supply channel-ordered PSFs; repeat for each channel.
--decon-scan-upsample VALUE Experimental 3D scan upsampling; requires deconvolution and full-volume GPU memory.
--eager-mode Enable eager stopping for planar deconvolution.
uv run process "/path/to/acquisition" --deconvolve --flatfield-correction

Append --help to list all options and defaults.